Tubular cells might be accessed by complexes of DNA reaching the kidney through the vasculature in one of two ways. The glomerular filtration barrier is the initial step in the process of entering the urine area. DNA complexes might depart the glomerular vascular system to get into tubular cells and go to the peritubular capillaries. The development of kidney-safe non-viral vectors would be made more accessible if the features of the complexes influencing the choice of delivery method could be clearly defined. Following systemic injection to laboratory animals, DNA complexes tagged with fluorescent may be utilized to track the organ dispersion of these particles, however up until now, little was known about the kidney. We used FITC-pLys (fluorescein isothiocyanate conjugated poly-l-lysine) as a commercially accessible fluorophore for both polyplexes and lipo-polyplexes. Pre-condensation of DNA with 0.4 N/P eq of FITC-pLys was used to make fluorescent complexes, which were then fully condensed with either PEI or DOTAP to produce polyplexes or lipo-polyplexes. Transfection efficacy in cultivated cells may be improved by using lipoplexes containing pLys.